Confocal Imaging Core

About Our Confocal Imaging Services

Confocal and Light Microscopy, Immunostaining, and Image Analysis

The function of the Confocal Imaging Core is to provide services in confocal and light microscopy, immunostaining and image analysis for cells and tissues including:

Confocal microscopy
Bright-field and fluorescence microscopy
Immuno-peroxidase (IHC-DAB) and immuno-fluorescence (IF) staining
Image Analysis for bright-field and fluorescence images

Find a Researcher

Fee Structure

Equipment

Getting Started, Procedures & Policies

Contact our Core Staff to schedule an an initial meeting.
During this meeting, the project and experimental details will be discussed. Core staff could suggest which immunostaining technique, microscopy modality and analysis method works best for the project as well as consultation on strategies to accomplish the task. Users have the option of choosing service work or independent use.

Service Work: Core staff provides technical assistance and support to stain, acquire and analyze samples for the users. This service is most suited for users that use core equipment infrequently, and prefer hands-on assistance and support for their staining, image acquisition and analysis.

Independent Use: All users who wish to use microscopes and image analysis software independently are required to receive a formal training from the core staff first. Upon completion of the training session, users will be permitted to operate the equipment independently, and will be given a user ID and password to make reservation on the online calendar. Immunostaining is provided only as a service.
Schedule a service work session or a customized training session with one of the core staff members.

Print and complete the Core Service Order Sheet and send it with your request. Please mention the type of service required. For immunostaining, mention the type of staining required: immunofluorescence (IF) or immunoperoxidase (IHC-DAB), the antibodies of interest and the number of slides to be stained for those antibodies. You can mention details under the Comments/Special Instructions section. Please fill out the form in its entirety and enter Grant # (or Dept ID) for internal (BIDMC) users and PO# for external users.

Microscope Policies

How To Acknowledge

To ensure continued support from NIH and our hospital, we request that you acknowledge our core facility if we have made contribution to your publication. In your acknowledgement, please reference us as the Confocal Imaging Core Facility at BIDMC. If you are an HDDC member, please cite the center grant imaging core B, DK-034854, on all publications.